214 research outputs found
Recreation Demand of Consumer with Experiential Marketing in Festival
AbstractExperiential marketing that allows visitors to fully participate and appreciate festival activities con be coupled with strategies of market segmentation, which is of great benefit to industrial and cultural activities. In this study, we used factor analysis method to understand the perception factor of visitors participate the Sweet Taiwan Year Festival in Tainan country of Taiwan. And segmented festival market by cluster analysis based on delineated experiential perception factors. The empirical result of this study shows that experiential perception clusters and the visitor type have become the most important factors in influencing the experiential value, the relation quality and the relation outcome
Clonal spread of SCCmec type IV methicillin-resistant Staphylococcus aureus between community and hospital
ABSTRACTThe staphylococcal chromosome cassette (SCC)mec types of 382 hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA) isolates in Taiwan were analysed over a 7-year period (1999â2005). There was an abrupt increase in SCCmec type IV in HA-MRSA during 2005. The molecular epidemiology of a subset (n = 69) of HA-MRSA isolates with SCCmec types III, IV or V was characterised and compared with that of community-acquired MRSA (CA-MRSA) (n = 26, collected during 2005). Pulsed-field gel electrophoresis revealed three major pulsotypes (A, B and C) and 15 minor clones. Pulsotypes B and C, which contained isolates carrying SCCmec types IV and V, respectively, included both CA-MRSA and HA-MRSA isolates. Among 24 toxin genes analysed, five genes had significant differential distribution between CA-MRSA and SCCmec type III HA-MRSA. Furthermore, among SCCmec type IV isolates, the seb gene was detected more commonly in HA-MRSA. Analysis of representative members of the three major pulsotypes by multilocus sequence typing revealed two sequence types (STs), namely ST239 (SCCmec III) and ST59 (SCCmec IV or SCCmec V). This suggests that ST59:SCCmec IV, which is usually community-acquired, has become an important nosocomial pathogen in the hospital studied
A study of genetic variations, population size, and population dynamics of the catadromous Japanese eel Anguilla japonica (Pisces) in northern Taiwan
Japanese eels are widely distributed in northeast Asian countries, and they have a catadromous life history. In this article, we explored whether Japanese elvers have temporal genetic structure and whether the population went through population expansion during the Pleistocene. In total, 273 specimens were collected from the Tanshui River estuary, northern Taiwan, in 1989-2008. The highly variable region of the mitochondrial DNA D-loop was cloned and sequenced. A genealogy was reconstructed based on the Neighbor-joining method, and results showed an unobvious yearly clade and a high level of haplotype diversity, but low mean nucleotide diversity among samples. Most of the pairwise F (ST) appeared statistically insignificant. These genetic parameters suggested a lack of temporal population structure combined with a sustainable high effective population size of Japanese eels. Negative values of Tajima's D and Fu's F (s) appeared in all samples with high significance. The mismatch distribution, skyline plot, and minimum spanning network indicated that historical population expansion of the Japanese eel could be traced back to the Pleistocene. Results of this study imply the Japanese eel has a complex life history, and the temporal structure of Japanese eels should be continually monitored in the future
Comparison of Genomes of Three Xanthomonas oryzae Bacteriophages
<p>Abstract</p> <p>Background</p> <p>Xp10 and OP1 are phages of <it>Xanthomonas oryzae </it>pv. oryzae (Xoo), the causative agent of bacterial leaf blight in rice plants, which were isolated in 1967 in Taiwan and in 1954 in Japan, respectively. We recently isolated the Xoo phage Xop411.</p> <p>Results</p> <p>The linear Xop411 genome (44,520 bp, 58 ORFs) sequenced here is 147 bp longer than that of Xp10 (60 ORFs) and 735 bp longer than that of OP1 (59 ORFs). The G+C contents of OP1 (51%) and Xop411 and Xp10 (52% each) are less than that of the host (65%). The 9-bp 3'-overhangs (5'-GGACAGTCT-3') in Xop411 and Xp10 are absent from OP1. More of the deduced Xop411 proteins share higher degrees of identity with Xp10 than with OP1 proteins, while the right end of the genomes of Xp10 and OP1, containing all predicted promoters, share stronger homology. Xop411, Xp10, and OP1 contain 8, 7, and 6 freestanding HNH endonuclease genes, respectively. These genes can be classified into five groups depending on their possession of the HNH domain (HNN or HNH type) and/or AP2 domain in intact or truncated forms. While the HNN-AP2 type endonuclease genes dispersed in the genome, the HNH type endonuclease genes, each with a unique copy, were located within the same genome context. Mass spectrometry and N-terminal sequencing showed nine Xop411 coat proteins, among which three were identified, six were assigned as coat proteins (4) and conserved phage proteins (2) in Xp10. The major coat protein, in which only the N-terminal methionine is removed, appears to exist in oligomeric forms containing 2 to 6 subunits. The three phages exhibit different patterns of domain duplication in the N-terminus of the tail fiber, which are involved in determination of the host range. Many short repeated sequences are present in and around the duplicated domains.</p> <p>Conclusion</p> <p>Geographical separation may have confined lateral gene transfer among the Xoo phages. The HNN-AP2 type endonucleases were more likely to transfer their genes randomly in the genome and may degenerate after successful transmission. Some repeated sequences may be involved in duplication/loss of the domains in the tail fiber genes.</p
Sequence and phylogenetic analyses of the twin-arginine targeting (Tat) protein export system
Twin-arginine targeting (Tat) protein secretion systems consist of two protein types, members of the TatA and TatC families. Homologues of these proteins are found in many archaea, bacteria, chloroplasts and mitochondria. Every prokaryotic organism with a fully sequenced genome exhibits either neither family member, or between one and three paralogues of these two family members. The Arabidopsis thaliana genome encodes three of each. Although many mitochondrially encoded TatC homologues have been identified, corresponding TatA homologues have not been found in this organelle. Phylogenetic analyses reveal that most prokaryotic Tat systems consist of one TatC homologue and two sequence-divergent TatA homologues (TatA and TatB). When only one TatA homologue is present, TatB is missing, and when three TatA homologues are present, the third one arose by duplication of TatA, not TatB. Further, homologues most resembling TatB are more sequence-divergent than those more closely resembling TatA. In contrast to the TatA family, the TatC family shows phylogenetic clustering in strict accordance with organismal type. These results are discussed in terms of their probable structural, functional and evolutionary significance
The effects of the bacterial interaction with visible-light responsive titania photocatalyst on the bactericidal performance
Bactericidal activity of traditional titanium dioxide (TiO2) photocatalyst is effective only upon irradiation by ultraviolet light, which restricts the potential applications of TiO2 for use in our living environments. Recently carbon-containing TiO2 was found to be photoactive at visible-light illumination that affords the potential to overcome this problem; although, the bactericidal activity of these photocatalysts is relatively lower than conventional disinfectants. Evidenced from scanning electron microscopy and confocal Raman spectral mapping analysis, we found the interaction with bacteria was significantly enhanced in these anatase/rutile mixed-phase carbon-containing TiO2. Bacteria-killing experiments indicate that a significantly higher proportion of all tested pathogens including Staphylococcus aureus, Shigella flexneri and Acinetobacter baumannii, were eliminated by the new nanoparticle with higher bacterial interaction property. These findings suggest the created materials with high bacterial interaction ability might be a useful strategy to improve the antimicrobial activity of visible-light-activated TiO2
Text Line Segmentation of Historical Documents: a Survey
There is a huge amount of historical documents in libraries and in various
National Archives that have not been exploited electronically. Although
automatic reading of complete pages remains, in most cases, a long-term
objective, tasks such as word spotting, text/image alignment, authentication
and extraction of specific fields are in use today. For all these tasks, a
major step is document segmentation into text lines. Because of the low quality
and the complexity of these documents (background noise, artifacts due to
aging, interfering lines),automatic text line segmentation remains an open
research field. The objective of this paper is to present a survey of existing
methods, developed during the last decade, and dedicated to documents of
historical interest.Comment: 25 pages, submitted version, To appear in International Journal on
Document Analysis and Recognition, On line version available at
http://www.springerlink.com/content/k2813176280456k3
Charmless Decays Based on the six-quark Effective Hamiltonian with Strong Phase Effects II
We provide a systematic study of charmless decays (
and denote pseudoscalar and vector mesons, respectively) based on an
approximate six-quark operator effective Hamiltonian from QCD. The calculation
of the relevant hard-scattering kernels is carried out, the resulting
transition form factors are consistent with the results of QCD sum rule
calculations. By taking into account important classes of power corrections
involving "chirally-enhanced" terms and the vertex corrections as well as weak
annihilation contributions with non-trivial strong phase, we present
predictions for the branching ratios and CP asymmetries of decays into
PP, PV and VV final states, and also for the corresponding polarization
observables in VV final states. It is found that the weak annihilation
contributions with non-trivial strong phase have remarkable effects on the
observables in the color-suppressed and penguin-dominated decay modes. In
addition, we discuss the SU(3) flavor symmetry and show that the symmetry
relations are generally respected
The performance of the jet trigger for the ATLAS detector during 2011 data taking
The performance of the jet trigger for the ATLAS detector at the LHC during the 2011 data taking period is described. During 2011 the LHC provided protonâproton collisions with a centre-of-mass energy of 7 TeV and heavy ion collisions with a 2.76 TeV per nucleonânucleon collision energy. The ATLAS trigger is a three level system designed to reduce the rate of events from the 40 MHz nominal maximum bunch crossing rate to the approximate 400 Hz which can be written to offline storage. The ATLAS jet trigger is the primary means for the online selection of events containing jets. Events are accepted by the trigger if they contain one or more jets above some transverse energy threshold. During 2011 data taking the jet trigger was fully efficient for jets with transverse energy above 25 GeV for triggers seeded randomly at Level 1. For triggers which require a jet to be identified at each of the three trigger levels, full efficiency is reached for offline jets with transverse energy above 60 GeV. Jets reconstructed in the final trigger level and corresponding to offline jets with transverse energy greater than 60 GeV, are reconstructed with a resolution in transverse energy with respect to offline jets, of better than 4 % in the central region and better than 2.5 % in the forward direction
Measurement of the cross section for isolated-photon plus jet production in pp collisions at âs=13 TeV using the ATLAS detector
The dynamics of isolated-photon production in association with a jet in protonâproton collisions at a centre-of-mass energy of 13 TeV are studied with the ATLAS detector at the LHC using a dataset with an integrated luminosity of 3.2 fbâ1. Photons are required to have transverse energies above 125 GeV. Jets are identified using the anti- algorithm with radius parameter and required to have transverse momenta above 100 GeV. Measurements of isolated-photon plus jet cross sections are presented as functions of the leading-photon transverse energy, the leading-jet transverse momentum, the azimuthal angular separation between the photon and the jet, the photonâjet invariant mass and the scattering angle in the photonâjet centre-of-mass system. Tree-level plus parton-shower predictions from Sherpa and Pythia as well as next-to-leading-order QCD predictions from Jetphox and Sherpa are compared to the measurements
- âŠ